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Recombinant DNA Technology and Genetic Engineering
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About Recombinant DNA Technology and Genetic Engineering

Genetic engineering (also called genetic modification) is a process that uses laboratory-based technologies to alter the DNA makeup of an organism. This may involve changing a single base pair (A-T or C-G), deleting a region of DNA or adding a new segment of DNA. For example, genetic engineering may involve adding a gene from one species to an organism from a different species to produce a desired trait. Used in research and industry, genetic engineering has been applied to the production of cancer therapies, brewing yeasts, genetically modified plants and livestock, and more.

Genetic engineering, also called genetic modification or genetic manipulation, is the modification and manipulation of an organism's genes using technology. It is a set of technologies used to change the genetic makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms. New DNA is obtained by either isolating or copying the genetic material of interest using recombinant DNA methods or by artificially synthesizing the DNA. A construct is usually created and used to insert this DNA into the host organism. The first recombinant DNA molecule was made by Paul Berg in 1972 by combining DNA from the monkey virus SV40 with the lambda virus. As well as inserting genes, the process can be used to remove, or "knock out", genes. The new DNA can be inserted randomly, or targeted to a specific part of the genome.

Importance of Recombinant DNA Technology and Genetic Engineering

Genetic modification allows changes in genes to transfer genetic material in different organisms. It is possible to introduce foreign genes into crop plants and express these in specific tissues. Transfer of genes can help to improve crop protection. Genetic modification can be done with various viral and non viral methods. Direct manipulation of DNA sequences can lead to different expression levels. Choosing the best method for gene delivery can be a difficult task, particularly to transfer genetic material in a proper way. This review highlights, basic mechanism and importance and different methods used for efficient gene delivery. Hence, understanding of intracellular machinery plays a crucial role for designing a more effective gene delivery environment.

Job Scope

Jobs directly related to your degree include:

Academic researcher

Biomedical scientist

Clinical research associate

Clinical scientist, genomics

Clinical scientist, immunology

Genetic counselor

Plant breeder/geneticist

Research scientist (life sciences)

Research scientist (medical)

Jobs where your degree would be useful include:

Biotechnologist

Data scientist

Epidemiologist

Forensic scientist

Medical science liaison

Physician associate

Scientific laboratory technician

Science writer

Secondary school teacher



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